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Pradheep Chhalliyil



Dr. Pradheep Challiyil is a scientist, educator and an author of several books.

He is now a Biotechnology consultant working to solve challenges in the application of RNA, DNA for molecular biology applications like qPCR, Digital PCR, DNA sequencing etc. 

Dr. Chhalliyill has 30 plus years of professional scientific experience. In 1997, Dr. Challiyil co-founded a 503(c) tax-exempt non-profit organization called Sakthi Foundation. He also started a Sakthi Schools, a subsidiary of Sakthi Foundation. Today, there are several Sakthi Schools operation in Southern India. In addition, he developed a new effective, integrated and holistic education system known as the Uni5 system of education. In fact, all the Sakthi Schools, as well as few other schools in India, are adopting this Uni5 system of education.

Sakthi Foundation website will provide an immense amount of information ranging from health, happiness, relationship, pattern-based education, parenting, energy, and spirituality. He has delivered numerous lectures all around the globe. He also provides advice through Uni5 telegram groups on the above topics.





A detail-oriented and results-driven individual with over 19 years of experience as a lead player in R&D, developing technologies for detecting Food adulteration and GMO (Genetically modified organisms). Highly competent and driven biotechnology professional with exceptional analytical and problem-solving abilities. Considerable expertise in database architecture, medical informatics, and computational biology methodologies and common laboratory safety practices. Adept at collecting accurate information comparing several types of research data and engaging in medical and food research with corporate compliance work. High energy, dedicated professional, committed to practical and theoretical teaching, increasing productivity, and maintaining quality and efficiency.


  • Biotechnology
  • Quality Assurance/Testing
  • Statistical Analysis
  • Workflow Streamlining
  • Technical Communications
  • Instrumentation
  • NextGen Sequencing
  • Molecular and Cell Biology
  • Marketing Strategies
  • Research Services
  • Assay Development
  • Team Leadership/ Motivation
  • Project Management
  • Conflict Resolution


Co-Founder & Chief Scientific Officer                                                                                       March 2021-Present
Accurate Molecular Mutation Analytical Genomics (AMMAGENOMICS), Chennai, India  
Developing Technologies (Patent Pending) for Biotechnology, Medical, Pharmaceutical and Food Industry

CSO & Founder                                                                                                                  November 2021-Present

Oorjv Certification of Food and Herbal Medicines for quality and verification

Chief Scientific Officer                                                                                                           November 2021 -Present

YSA Labs, Tempe, Arizona, USA

Developed Technology and Patent Pending RNA Extraction Free Technology

Biotechnology Consultant                                                                                             August 2019-2020 (1 year)

SomaGenics, Inc. CA, USA                                                                                                                                                                                                        

Developed SNP assay

Molecular Diagnosis Director                                                                                          July 2020-January 2021

Diagnostic Lab Chennai, T.Nadu, India                                                                                      

Evaluated and implemented new technologies and laboratory techniques to facilitate high-throughput data acquisition.

Senior Scientist                                                                                                             April 2000- Jan 2019

Foodchain ID NA INC                                                                                                                                                                                                                    

19 years of experience as a Lead player in R&D, developing technologies for detecting GMO (Genetically modified organisms) allergens and monitoring food adulteration using qPCR and Digital PCR and sequencing methods, which were used by the company’s all branches and franchising units all over the globe.

Postdoctoral Fellow                                                                                                                                   1997-2000

Roswell Park Cancer Center                                      

Researched the cause of recurrence of cancer in patients who received chemotherapy for Leukemia.

Lecturer                                                                                                                                               1990-1992

PSGCAS, Coimbatore, India                                                                                                                              

Took the initiative in creating the course syllabus and establishing the curriculum for graduate and undergraduate students studying biochemistry and biotechnology.


  • RNA and DNA High-Quality Extraction India, USA 2022-Patent Pending
  • RNA single tube Extraction free Method USA 2021-Patent Pending
  • A process for the isolation of the anticancer principle from medicinal plants (Cleistanthin) - India - 1539/MAS/95.
  • An electrochemical process for water sterilization by singlet Oxygen production - 1622/MAS/95.


Madurai Kamaraj University, India

Ph.D., Effects of Cleistanthins on the Proliferation of Normal and Tumor Cells                                                           1998

Doctorate in Biochemistry & Cancer Biology                                                                                                        1992-1997

PSGCAS is affiliated with Bharathiyar University, Coimbatore, India

Masters in Biochemistry                                                                                   1988

Bachelors                                                                                                        1985


  • Skilled in Primer Probe design to detect even single base pair change (point mutations) that helps in the detection of specific gene targets with high specificity and sensitivity.
  • Skilled in developing Nucleic acid (RNA & DNA) extraction using Magnetic beads, spin columns, and direct PCR from all human samples, crops, plants, animals, insects, microbes, and soil.
  • Skilled in developing diagnostic methods for clinical, research, environmental, and other applications.  
  • Skilled in developing methods for Next-Gen Sequencing, metagenomics, and chromosome walking for flanking regions from all biological samples.
  • Skilled in developing inhibition-free Master-mix, Digital PCR, Multiplex PCR, LAMP, and Chip-based technologies.
  • Developed technologies to detect food adulteration, safety, assay for antioxidants, polyphenols, food components.
  • Analytical and problem-solving skills.
  • Multitasking abilities. 


Co-Founder / Leaders (AMMAGENOMICS) Accurate Molecular Mutation                    11 November 2020-Present

Analytical Genomics                            

  • Developing AMMAGENOMICS, a startup, to become one of Chennai's top 10 COVID testing labs in six months with a successful turnover that employs more than 50+ employers.
  • Being a leader in the development of biotechnology products and services for a variety of industries, including the industrial, diagnostic, and testing sectors.
  • Directing effective technical training for lab and professional training courses, internships, and externships.
  • Using creative pattern-based teaching to lead the Uni5 School's curriculum development.
  • Gave health tips for various diseases to thousands of people all over the globe using emails, web sites, social media, health awareness campaigns, talks etc.
  • Amassing more than 24 million hits on the website in 10 years.
  • Using public speeches and literature to guide kids and adults through the Selftual (life purpose) questions.
  • Directing the development of several food products that are marketed on and are both nutritional and therapeutic.
  • Technologies were created and put into use by sales and management teams to provide quick turnaround of samples for business development.


In Press 

  • Pranav Chhaliyil1, Pradheep Chhalliyil, Bernd Schoel Effect of compost on soil Microbiota
  • Pradheep Chhalliyil   et al Adulteration in Milk and diary products in the Market
  • Pradheep Chhalliyil  et al High Quality Microbiota Profile in Stool Samples using AMMAGENOMICS Microbial DNA Kit
  • Pradheep Chhalliyil  et al Polyphenol Free DNA and RNA from plant samples using AMMAGENOMICS Plant DNA Kit
  • Pradheep Chhalliyil  et al Quick Saliva DNA analysis using AMMAGENOMICS Saliva DNA Kit
  • Pradheep Chhalliyil  et al  High sensitive Viral DNA and RNA detection in Blood Plasma samples using AMMAGENOMICS Blood DNA RNA Kit
  • Pradheep Chhalliyil  et al  Metagneomics Profile of Soil Microbiota using AMMAGENOMICS Soil DNA Kit
  • Pradheep Chhalliyil  et al  Enhanced Gram positive Bacteria Profiling using AMMAGENOMICS Microbial DNA Kit
  • Pradheep Chhalliyil  et al Blood Fungal and Yeast detection made easy and efficient using AMMAGENOMICS Fungal DNA Kit
  • Pradheep Chhalliyil, Usha Nandini, Neelima thota. Skin Diseases Cured: a case report Submitted

TEACHING EXPERIENCE                                                                                                                        1990 to 1997

Lecturer in Biochemistry, P.S.G.College of Arts & Sciences, CBE.                                       December 1990 – May 1992

Biochemistry theory & practicals for MSc Biology and Biotechnology at M. K. University (1992 -97).


UGC Lectureship 1990 (University Grants Commission)

ICAR Biotechnology 1991 (Indian council of Agri. Research)

UGC-CSIR JRF 1992 (Council of Scientific & Industrial Research).

UGC-CSIR SRF 1994 (Council of Scientific & Industrial Research).


 The first “Anna Memorial GOLD AWARD”, for the best Ph.D. thesis published in the cancer field (India).

Winner-Book Award 2004at Hollywood Spiritual Film Festival


Incompatible Foods (Virudh Aaahar) English September 2022

Deivamum Deiveega Sakthigalum: Tamil   (August 11, 2020)

Journey to the Source, Decoding Matrix Trilogy, Sakthi Books 2004, Iowa.

“Putrunoyyium Unavum” (Diet & Cancer- Co-author Dr.M.Madeswaran) Tamil, 1997, Maruthi Publishers, Coimbatore, India.

"Bhakshanavum Cancerum" Malaylam, Sakthi Foundation 2013, USA - Co-Author-Dr.M.Thomas).

25 million view hits with more than  2000 pages of information on the web on Health, Relationships, Parenting, Education, Spirituality, Selftuality, and Community Responsibility.


  • First International Comprehensive Cancer Care conference: Center for mind-body medicine. 12-14th June 1998, Washington DC, USA (Full scholarship was given to attend the conference).
  • First International Congress on Tibetian Medicine: 7-9th November 1998, Washington DC, USA (Full scholarship was given to attend the conference).
  • 1st Global Conference on GMO Analysis, Villa Erba, Como, Italy, 24-27 June 2008, GM rice screening in the Mexican market by real-time PCR.
  • Pittcon Conference 2007, 2009, 2012, 2016 8. PAG Plant Animal Genome conference – 2008, 2012-2019 9. Real-Time Conference - 2007 • G-OnRamp Workshop Agenda 2018 Washington University in St. Louis, July 16-19 
PO0203 In vitro Effect of Sugarcane Polyphenols on Oral Microbiota
Pranav Chhalliyil, Pradheep Chhalliyil, and Bernd Schoel 
P1204 A Rapid Method for Extraction and Purification of DNA from Dental Plaque, Tongue Scraping and Saliva Using IDeal Kit 2017 INTLPAG.ORG
Pranav Chhalliyil, Pradheep Chhalliyil, and Bernd Schoel, Genetic ID NA, Inc, Fairfield, IA 
P0206 IDeal Fast ID Kit for DNA Extractions from Saliva Samples. Jan 9-13 2016 INTLPAG.ORG Pranav Chhaliyil, Genetic-ID; Bernd Schoel, Pradheep Chhalliyil
P0216 High PCR Efficiency Using Ideal DNA Extraction Kit an 9-13 2016 INTLPAG.ORG Pradheep Chhalliyil, Bernd Schoel, Genetic ID NA, Inc 
P0061 Quantitative Digital and Real Time PCR for GM Alfalfa January 12, 2015 Pradheep Chhalliyil, Bernd Schoel , Genetic ID NA, Inc, Fairfield, IA 
P0045 High PCR Efficiency Using FASTMAG DNA Extraction Kit January 12, 2015 Pradheep Chhalliyil, Bernd Schoel , Genetic ID NA, Inc, Fairfield, IA


LinkedIn Professional Comments: (REVIEWS)

John Fagan Ph.D.John Fagan Ph.D.· 1stFirst degree connection

Chairman and Chief Scientist at Health Research Institute - HRI LabsChairman and Chief Scientist at Health Research Institute - HRI Labs September 14, 2020, John managed Pradheep directly September 14, 2020, John managed Pradheep directly

While I was Chairman and Chief Scientific Officer at Genetic ID/Global ID Group (now FoodChain ID), Pradheep played a key role in technology development over many years. He was consistently creative and innovative, thinking outside the box to deliver solutions that were often much better than what was expected. His innovations in PCR and DNA extraction saved the company millions of dollars throughout his tenure at the company. I considered him a key corporate asset that added substantial value to the company. He is also highly idealistic and has continuously worked as a leader in poverty removal projects in his native India, giving his time and resources to improving the quality of life for rural people. He is also easy to work with, cooperative and helpful to colleagues, and has excellent communication skills. Highly recommended on all counts!

Brian H. JohnstonBrian H. Johnston· 1stFirst degree connection

Founder and CEO, SomaGenics, Inc. Founder and CEO, SomaGenics, Inc. November 6, 2019, Brian H. managed Pradheep directly November 6, 2019, Brian H. managed Pradheep directly

I recently met Pradheep through a friend and collaborator, and on his recommendation, I engaged Pradheep to help solve a very challenging genomics problem. His ideas for solutions were spot-on and within a few weeks, he had solved the problem, generated all the proof of concept data, and documented his work clearly and comprehensively. He is very knowledgeable, resourceful, and efficient in designing and executing experiments. He is also a delight to work with, considerate, cheerful, and always ready to chat about science.

Bill Thompson· 1stFirst degree connection

Chief Executive Officer Chief Executive Officer August 2, 2011, Bill was senior to Pradheep but didn't manage Pradheep directly August 2, 2011, Bill was senior to Pradheep but didn't manage Pradheep directly

Pradheep is an exceptional scientist. He embodies a rare combination of deep intellectual and analytical abilities combined with a highly creative and intuitive approach to problem-solving very complex scientific challenges. His highly desirable skill set makes him an ideal person to have on board one's Research and Development Team.
Over the 7 years, I worked with Pradheep. I have witnessed him succeed in achieving the goals set for him time after time. This included developing breakthroughs in GMO testing methods and establishing new technologies for the company. Additionally, Pradheep as a person is wonderful to work with. His personality and his positive attitude to work and life make him a complete pleasure to interact with daily.

 Short CV

Scientist interested in solving  challenges all walks of life. 

prad 2016 maySocial responsibility:   Co-founded and works at free time for a community-profiting organization (Uni5-SakthiFoundation INC).

Co-started a five level integrated Holistic Education Uni5 Schools in India.

Gives awareness talks on aspects of life (Health, Relationships, Parenting, Education, Spirituality, Selftuality and Community Responsibility).

Ph.D in Cancer Biology, Madurai Kamaraj University, Madurai, India

  • Post doctoral Fellow, Roswell Park Cancer Institute, Buffalo, NY.
  • M. S. Biochemistry PSGCAS, Coimbatore (India)
  • B. S. Biochemistry PSGCAS, Coimbatore (India)

Importance of Ph.D work in Cancer : This work was the first to show the effect of Clesitanthin compounds purified from cleistanthus collinus on cancer cells with least effect on normal cells in comparison to other leading cancer drugs. Cleistanthus collinus is a toxic plant , but purification results in removing the toxic effects and showing anticancerous activity.

This work is still motivating other researchers to work on these compounds.  Godvari Refinieries ,  Sathgen,  a company has studied the effect on clesitanthin and its derivatives. 

A pub med search

Ph.D Work: Cleistanthin as Anti-cancer agent

News in "The Hindu"

News in Tamil Paper

News in Malayalam Paper

Work quoted in Book


ALTERNATIVES TO THE USE OF LIVE VERTEBRATES IN BIOMEDICAL RESEARCH AND TESTING : A bibliography with Abstracts: Prepared by NATIONAL LIBRARY OF MEDICINE NATIONAL INSTITUTES OF HEALTH BETHESDA, MD Vera W. Hudson, M.S. Project Coordinator and Scientific Editor, National Library of Medicine and Elizabeth DeBerry, Copy Editor, National Library of Medicine: 1997 No. 3.

Cytotoxic and genotoxic effects of cleistanthin B in normal and tumor cells. Prabhakaran C Kumar P, Panneerselvam N, Rajesh S, Shanmugam G.

2. Quick Tips for Balanced Living:Himalayan Institute Press-2005. Inner Cleansing by Pradheep Chhalliyil -Page 32-34.

3. From Superman to Brahman: The Religious Shift of The Matrix Mythology    

Journal of Religion and Film, Jeffery Wittung, Daniel Bramer Volume 10 Issue 2 October 2006



 Co-Founded Uni5 Sakthi Foundation INC

  • A Tax exempted Non-Profit (Community Profiting) organization operating in USA, India, and Indonesia and doing various charitable activities.
  • Giving health information to hundreds of people all over the globe for various ailments.
  • Help to fund underprivileged children, orphans for their school education.
  • Bring awareness among underprivileged people through health camps, such as “eye camps”, and diabetic camps in remote areas of Tamil Nadu.
  • Create Audiovisual materials to help create health awareness.
  • Started the first free or pay-as-capable Uni5schools in Sendurai (Madurai), Coimbatore, Porayar (as opposed to middle/upper-class oriented schools in urban locations). (
  • Support the work of other non-profit (NGO's) organizations.



    • UGC Lectureship 1990 (University Grants Commission)
    • ICAR Biotechnology 1991 (Indian council of Agri.Research)
    • UGC-CSIR JRF 1992 (Council of Scientific & Industrial Research).
    • UGC-CSIR SRF 1994 (Council of Scientific & Industrial Research).

Links to Talks

Topics of Talks

1. Health and luck factor !. More modern scientific research shows that more than genetics, environment and life style, diseases like cancer is caused by randomness. The talk will be about luck in life, how to understand it and how to increase it. The content is totally different from the common views of information not thought about by doctors or health professionals.

2. Successful Parenting !. This topic is about the five needs of a child that is taken care from the young age will guarantee success in a child's life. This will help to avoid stress both in the parents and the child. This is based on practically tried and found effective.

3. Happy and successful Carreer !.This talk helps adults and students to find the job enjoyable and successful. It also addresses what career one should choose and find happiness in all five levels of being.

4. Simplified Bhagavad Gita !.One of the most practical solutions for all day to day life problems we encounter. This one hour talk will give you the essence of Gita that you it will make sense the geniousness of the Ancient Indian thought on encountering life situations.

5. Can one Education pattern be effective for effective personal and professional life !. This talk brings evidence how a pattern based education can help all students, adults about a pattern based learning system that will make one brilliant in arts, science, maths and at the same time understand the mystery of oneself and the Universe.

Chhalliyil, P.; Ilves, H.; Kazakov, S.A.; Howard, S.J.; Johnston, B.H.; Fagan, J. A Real-Time Quantitative PCR Method Specific for Detection and Quantification of the First Commercialized Genome-Edited Plant. Foods20209, 1245.

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